Flow cytometry detection of apoptotic changes of U87-EGFRvIII cells following c-Met gene silencing. (A–D) Flow cytometry results of U87-EGFRvIII apoptosis in each group. Q2 and Q4 show late and early apoptotic rates, respectively. (E) Comparison of apoptotic rate of U87MG and U87-EGFRvIII cells in each group. BA, 5′-biotin-labeled aptamer 32; FITC, fluorescein isothiocyanate; siRNA, small interfering RNA; lipo + ncRNA + s = liposome + control siRNA + streptavidin. lipo + siRNA + s = liposome + c-Met siRNA + streptavidin; BA + ncRNA + s = BA + control siRNA + streptavidin; BA + siRNA + s = BA + c-Met siRNA + streptavidin. Error bars show the means ± standard deviation (n=3). *P<0.05, **P<0.01.