Table 2. Kinetic parameters of PfSHMT variants.

The K _m of the C125P variant was not determined (ND) owing to low catalytic activity.

	K m (mM)
Protein	L-Serine	THF	k cat (s−1)
WT	0.123 ± 0.019	0.086 ± 0.005	3.71 ± 0.04
F292E	0.112 ± 0.004	0.077 ± 0.002	3.61 ± 0.16
C125P + dithiothreitol	ND	ND	0.32 ± 0.14
C125P − dithiothreitol	ND	ND	0.24 ± 0.06
C364A + dithiothreitol	0.067 ± 0.008	0.028 ± 0.002	4.00 ± 0.01
C364A − dithiothreitol	0.070 ± 0.008	0.038 ± 0.006	3.91 ± 0.15
C364S + dithiothreitol	0.074 ± 0.007	0.150 ± 0.021	3.77 ± 0.08
C364S − dithiothreitol	0.082 ± 0.019	0.135 ± 0.019	3.84 ± 0.28