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The stability of the [2Fe–2S] cluster of NAF-1 H114C is greatly increased. The cluster stabilities of NAF-1 (blue), NAF-1 H114C (red) and Fd (green) were monitored over time by optical spectroscopy at 458 nm, which is attributed to cluster absorbance at 37°C. The absorbance decay corresponds to loss of the [2Fe–2S] cluster. The cluster is much more stable in NAF-1 H114C compared with native NAF-1 protein and its stability is almost equal to that in Fd.
