Table 2. Cellular ATP levels after incubation with TNFα in the presence or absence of adenosine.
Incubation Time (h) | |||
Treatment | 24 | 48 | 72 |
Control | 11.4±0.9 | 12.5±0.8 | 11.6±1.3 |
TNFα (1 ng/ml) | 9.6±0.1* | 10.5±0.8* | 9.3±0.7* |
Ado (10 µM) + TNFα | 10.8±0.7 | 11.7±1.0 | 12.1±1.8 |
HMEC-1 cells were incubated in the presence or absence of TNFα or TNFα+Ado (adenosine) for the indicated durations, then lysed and total ATP levels (nmol/106 cells) were measured as described in Methods. Values are means ± S.D. from 3 separate experiments. *denotes significantly different from respective control value.