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. 2014 Jun 10;9(6):e98459. doi: 10.1371/journal.pone.0098459

Table 2. Cellular ATP levels after incubation with TNFα in the presence or absence of adenosine.

Incubation Time (h)
Treatment 24 48 72
Control 11.4±0.9 12.5±0.8 11.6±1.3
TNFα (1 ng/ml) 9.6±0.1* 10.5±0.8* 9.3±0.7*
Ado (10 µM) + TNFα 10.8±0.7 11.7±1.0 12.1±1.8

HMEC-1 cells were incubated in the presence or absence of TNFα or TNFα+Ado (adenosine) for the indicated durations, then lysed and total ATP levels (nmol/106 cells) were measured as described in Methods. Values are means ± S.D. from 3 separate experiments. *denotes significantly different from respective control value.