Table 1. Photoconversion parameters of Dendra2.
PVA film | Mammalian cells | |
Photoconversion brightness (561 nm) [M−1cm−1] | (0.9±0.2)×10−5 | (0.9±0.4)×10−5 |
Photoconversion brightness (405 nm) [M−1cm−1] $ | (4.1±2.3)×10−1 | (1.4±0.6)×10−1 |
Photoconversion yield (405 nm) $ # | (4.0±2.3)×10−5 | (1.4±0.6)×10−5 |
Mean values ± SD are shown. Number of samples per experiment n = 3.
: The high SDs are attributed to inhomogeneity and weakness of the 405 nm laser beam.
: Assuming an extinction coefficient ε405 = 10250 Mol−1 cm−1.