Figure 1. The human hair lysate obtained from the OM male donor inhibited the proliferation of early and late-passage human melanoma cells.

Gamma irradiation-resistant melanoma sublines generated from these cells remained susceptible to the inhibitory effects of the hair lysate. The viable cell count was evaluated in 3-day cultures of MeW187 melanoma cells plated at 13×10³ cells/well with or without the lysate following 3 previous passages (a); 4-day cultures of the MeW100 melanoma cells plated at 7×10³ cells/well following 38 previous passages (b); 4-day cultures of the Mew100 subline pre-selected by irradiation with a ¹³⁷Cs source (30 Gy) and plated at 10×10³ cells/well (c); 3-day cultures of FSTLC melanoma cells plated at 15×10³ cells/well after 53 passages (d); and 3-day cultures of the FSTLC subline preselected by irradiation (60 Gy) and plated at 15×10³ cells/well (e). Fresh medium with or without the lysate was added to the cultures once daily. The groups of cultures treated with the lysate were compared to the control cultures using Dunnett’s test. Arrows mark the initial cell number in the cultures, and the bars represent the mean values ± s.d. of the viable cell count in triplicate cultures. Flow cytometry analysis of CFSE-labelled cells demonstrated that there were fewer cell divisions in the melanoma cell cultures supplemented with the lysate than in the control group (f). The histograms of the CFSE dilutions in MeW155 melanoma cells cultured for 5 days with or without the lysate are representative of triplicate cultures. The lysate was added only once at the start of the 5-day culture following labelling with CFSE. The proliferation indices (P.I.) were calculated as the proportion of the mean final cell yield to the initial cell number in the cultures. The effects of the lysate were evaluated in the prolonged passaged cultures (g). Proliferation indices were determined for MeW168 melanoma cells cultured with or without OM lysate on days 3 and 7 when the cells were passaged, and on day 11. Fresh medium containing the lysate at a concentration of 0.1% (m/v) was added on days 0, 3, 7, and 9.