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. 2014 Jun 10;9(6):e98834. doi: 10.1371/journal.pone.0098834

Figure 3. Increased HO-1 and autophagy alleviated liver damage.

Figure 3

(A) IR treatment increases the histopathologic changes of liver. Extensive areas of hepatocyte necrosis (N), vacuolation (V) and sinusoidal congestion were observed in the IR group. The Znpp+IR and Znpp+RIPC+IR group showed markedly higher hepatocyte necrosis accumulation (N. A) and vacuo(V), whereas the RIPC+IR group still showed large areas of normal liver architecture, similar to the sham group mice (original magnification 200×; insets 400×). (B) The mean injury score of the RIPC+IR group was significantly lower than the IR group according to Suzuki's histologic classification. The mean injury scores in the Znpp+IR and Znpp+RIPC+IR groups were significantly higher than in the IR and RIPC+IR groups (*P<0.05 compared with the IR group; #P<0.05 compared with the RIPC+IR group). (C) IR treatment increased serum aminotransferase levels in mice compared with controls. Compared with the IR group, serum aminotransferase levels were lower in the RIPC+IR group. The mean serum levels of ALT and AST were highest in the Znpp+IR and Znpp+RIPC+IR groups. There were no significant differences in aminotransferase levels in the Znpp+IR and Znpp+RIPC+IR groups (*P<0.05 compared with the IR group; #P<0.05 compared with the RIPC+IR group).