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. 2014 Jun 10;9(6):e99083. doi: 10.1371/journal.pone.0099083

Figure 4. Induction kinetics for MMP-9, MMP-10, MMP-12, IL-6, and NF-kappaBia in glomeruli from Alport mice and CD151 knockout mice.

Figure 4

Panel A. Glomeruli were isolated from CD151 knockout mice and Alport mice along with strain/age matched wild type littermates at the indicated ages using bead isolation. Total glomerular RNA was analyzed by real time RT-PCR using primers specific for the indicated transcripts. Each data point represents at least five independent samples. Significant differences when comparing the data from mutants with wild type littermates are denoted with asterisks (p<0.05). Note that IL-6 and NF-kappaBia did not reach significance likely due to a large variance in the data, but trended towards significance. Panel B shows that MMP-10 protein is induced in Alport glomeruli at both 4 and 7 weeks of age as determined by immunofluorescence analysis. Scale bar = 15 µm.