Figure 5. Purification of non ELPylated HA from transgenic leaves and seeds by His tag-based affinity chromatography.

Non ELPylated HAs from leaves (A) and seeds (B) were purified by the IMAC method described in the materials and methods section. Purified proteins were separated by 10% SDS-PAGE and then detected using Coomassie Brilliant Blue staining (left) or by an anti-c-myc monoclonal antibody (right).