Figure 10. Western blot analysis of purified HAs treated/untreated with PNGase F.

Purified HAs from leaves were deglycosylated using the commercial PNGase F enzyme described in the Materials and Methods section. PNGase F-treated and untreated proteins were then separated in 10% SDS-PAGE. Recombinant proteins were detected using an anti-c-myc monoclonal antibody. “−” and “+” indicate PNGase F-untreated and treated samples, respectively.