Figure 4.
Analysis of cell differentiation on titanium substrates. Alkaline phosphatase staining (A) and a quantitative assay (C) were carried out on the titanium surfaces. Expression levels of osteogenic-related differentiation genes and proteins (OCN, OPN, and alkaline phosphatase) were measured by real-time polymerase chain reaction and Western blot, respectively (B and D).
Notes: ##P<0.01 versus NT group; *P<0.05, **P<0.01 versus Mg30 group.
Abbreviations: NT, hierarchical hybrid micro/nanostructured titanium surfaces; Mg30, hierarchical hybrid micro/nanostructured titanium surfaces followed by magnesium ion implantation for 30 minutes; Mg60, hierarchical hybrid micro/nanostructured titanium surfaces followed by magnesium ion implantation for 60 minutes; OD, optical density; OCN, osteocalcin; OPN, osteopontin; ALP, alkaline phosphatase.