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Cellular uptake efficiency of 1,5-dihexadecyl N,N-diglutamyl-lysyl-L-glutamate (GGLG) and maleimide-modified (M-)GGLG liposomes in HeLa, HCC1954, MDA-MB-468, and COS-7 cells at 4°C. Cells were precooled at 4°C for 2 hours, and then the medium was exchanged with fresh Dulbecco’s Modified Eagle’s Medium (without serum) containing 72 μg/mL of liposomes for a further 2 hours incubation at 4°C.
Notes: Error bars represent the standard error of the mean; n=4; *P<0.01.
