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. 2014 Jun 5;9:2849–2861. doi: 10.2147/IJN.S58540

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© 2014 Li and Takeoka. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Influence of N-ethylmaleimide (NEM) on the cellular uptake of liposomes. Cells were preincubated with or without (control group) 0.01 nM NEM in serum-free medium for 10 minutes, and then followed by 2 hours incubation with 72 μg/mL of 1,5-dihexadecyl N,N-diglutamyl-lysyl-L-glutamate (GGLG) or maleimide-modified (M-)GGLG liposomes at 37°C.

Notes: Data represent the percentage of the cellular uptake efficiency by NEM inhibition to that of control group without NEM treatment in serum-free medium. Error bars represent the standard error of the mean; n=3; *P<0.01. 100% indicates the level of cellular uptake efficiency of liposomes in serum-free medium at 37°C.