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. 2014 Jun 5;9:2849–2861. doi: 10.2147/IJN.S58540

Figure 4.

Figure 4

Influence of endocytosis inhibitors on the cellular uptake of 1,5-dihexadecyl N,N-diglutamyl-lysyl-L-glutamate (GGLG) and maleimide-modified (M-)GGLG liposomes. HeLa, HCC1954, MDA-MB-468, and COS-7 cells were preincubated with each of the following endocytosis inhibitors: 10 µg/mL of chlorpromazine (CPZ), 10 µg/mL of nystatin (Nys), 1 mM of methyl-β-cyclodextrin (MβCD), 10 µg/mL of cytochalasin D (Cyto D), or 20 μM of Wortmannin (Wort) for 30 minutes, and subsequently incubated with 72 μg/mL of GGLG or M-GGLG liposomes in fresh Dulbecco’s Modified Eagle’s Medium (without serum) for 2 hours at 37°C.

Notes: Data represent the percentage of the cellular uptake efficiency of liposomes treated with endocytosis inhibitor to that of the control group without treatment. Error bars represent the standard error of the mean; n=4; *P<0.01. 100% indicates the level of cellular uptake efficiency of liposomes in serum-free medium at 37°C.