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. 2014 Jun 5;9:2849–2861. doi: 10.2147/IJN.S58540

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© 2014 Li and Takeoka. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Relative cellular uptake efficiency of 1,5-dihexadecyl N,N-diglutamyl-lysyl-L-glutamate (GGLG) and maleimide-modified (M-)GGLG liposomes after comprehensive inhibition of conventional endocytosis by preincubation of endocytosis inhibitors, including 4 µg/mL of chlorpromazine (CPZ), 4 µg/mL of nystatin (Nys), 0.4 mM of methyl-β-cyclodextrin (MβCD), 4 µg/mL of cytochalasin D (Cyto D), and 5 µM of Wortmannin (Wort) with HeLa, HCC1954, MDA-MB-468, and COS-7 cells in serum-free medium for 30 minutes at 37°C.

Notes: Data represent the percentage of the cellular uptake efficiency of liposomes treated with the mixture of endocytosis inhibitors to the control group without treatment. Error bars represent standard error of the mean; n=3; *P<0.01. 100% indicates the level of cellular uptake efficiency of liposomes in serum-free medium at 37°C.