Table S1.

Lipid composition, size, drug-to-lipid ratio, and drug encapsulation efficiency of 1,5-dihexadecyl N,N-diglutamyl-lysyl-L-glutamate (GGLG) and maleimide-modified (M)-GGLG liposomes

Liposome	Composition (molar ratio)	Size (nm)	D/L (μg/mg)	Encapsulation efficiency (%)
GGLG liposome	GGLG/chol/PEG-DSPE/PEG-Glu2C18 (5/5/0.03/0.03)	167.50±56.03	94.2	93.3
M-GGLG liposome	GGLG/chol/PEG-DSPE/Mal-PEG-Glu2C18 (5/5/0.03/0.03)	178.60±8.79	99.2	95.0

Note: No significant difference between the GGLG and M-GGLG liposomes was confirmed in terms of the physical properties described.

Abbreviations: chol, cholesterol; D/L, drug-to-lipid; DSPE, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine; Glu2C₁₈, 1,5-dioctadecyl L-glutamate; Mal, maleimide; PEG, poly(ethylene glycol).