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. 2014 Jun 10;9(6):e99411. doi: 10.1371/journal.pone.0099411

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© 2014 Bang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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2–4×10⁶ moDCs were stimulated with 1×10⁷ M. stadtmanae or M. smithii cells and medium (for control) over a period of 24 h. Subsequently, RNA was isolated according to manufacturer's protocol and reverse-transcribed. Relative quantification of expression of genes encoding HBD1 and LL37 were calculated with the LightCycler 480 Software in relation to house-keeping gene hprt.