Figure 6. Stopped-flow FRET measurements.

(A) The indicated HIV^eCFP/eYFP reporter particles were purified from the supernatant of transfected 293T cells and suspended in PBS. Dissociation in the presence of 0.05% TX-100 was monitored by FRET analysis using a stopped-flow setup as described in materials and methods. FRET signal intensities averaged from six individual measurements each are plotted in red; black lines indicate single (mature, immature) or double (MA-CA, MA-SP1) exponential fits to the data, yielding the kinetic constants summarized in Table 1. (B) Residual plots derived from the data shown in (A) are represented besides the respective graphs.