Table 1. Primary sequence of the tested oligonucleotides and the predicted melting temperatures (Tm).

Modification	Probe designation/Codon change	Sequence	Tm
LNA	WT (CGC)	5′-CGCCAAGCGGCTCAT-3′	68°C
	R882C (CGC->TGC)	5′-CGCCAAGCAGCTCAT-3′	66°C
	R882H (CGC->CAC)	5′-CGCCAAGTGGCTCAT-3′	66°C
	R882P (CGC->CCC)	5′-CGCCAAGGGGCTCAT-3′	67°C
	R882S (CGC->AGC)	5′-CGCCAAGCTGCTCAT-3′	66°C
BNA(NC)	WT (CGC)	5′-CGCCA+AGCG+GCTCATGTT-3′	70°C
	R882C (CGC->TGC)	5′-CGCC+A+AGCAG+CTC+ATGTT-3′	70°C
	R882H (CGC->CAC)	5′-CGCC+A+AGTG+GCTC+ATGTT-3′	70°C
	R882P (CGC->CCC)	5′-CGCCA+AGGGG+CTCATGTT-3′	70°C
	R882S (CGC->AGC)	5′-CGCC+A+AGCTG+CTC+ATGTT-3′	70°C

The character “+N” denotes the position of any BNA(NC) modified nucleotide. The positions of LNA modification are proprietary property of Exiqon, Denmark and are, therefore, not disclosed.