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. 2014 Jun 10;9(6):e99526. doi: 10.1371/journal.pone.0099526

Figure 5. Poly I:C pretreatment reduces oxygen-glucose deprivation (OGD)–induced astrocyte injury.

Figure 5

Astrocyte cultures were treated with 5 µg/mL or 10 µg/mL Poly I:C 24 h before OGD. (A and B) Poly I:C pretreatment reduced OGD-induced astrocyte injury as measured by MTT assay (A) and lactate dehydrogenase (LDH) release (B). (C and D) Western blot analysis showed that Poly I:C pretreatment increased the expression of TRIF protein (C) and pIRF3 protein (D) in astrocytes subjected to 12-h OGD. (E) ELISA showed that OGD alone significantly increased IFNβ release and that Poly I:C pretreatment increased IFNβ release further. (F) ELISA showed that OGD alone significantly increased IL-6 release but that Poly I:C pretreatment inhibited the increase in IL-6 release. Values are expressed as mean ± SD of three independent experiments, each carried out in triplicate; *p<0.05, **p<0.01.