Table 1. Significant temperatures (°C) for monoformat inulin isoforms.
AI-1 | AI-2 | GI | DI | ZI | EI | OI | |
---|---|---|---|---|---|---|---|
Tc/Conversiona | 17 | 32 | 45 | 54 | 60 | 64 | 71 |
Annealb | 5 | 20 | 20 | 37 | 37 | 45 | 45 |
Extractc | 25 | 39 | 49 | 57 | 62 | 68 | N/A |
1st wash | 0 d | 29 | 43 | 52 | 58 | 62 | 68 |
Assay (OD700)e | 10 | 21 | 39 | 49 | 55 | 60 | 68 |
80-100 mg mL-1, 90 min;
7-14 days;
40-50 mg mL-1;
All washes are at 0 °C;
T °C to distinguish that isoform from the next lower isoform.