Fig. 5.
Two pockets, one for recognition and one for incision, underlie Nfi mechanism. A) DNA schematic shows the relative position of the damage and the phosphodiester incision 3′ to the damage. B) The substrate structure (2w36.pdb) overlaid with the Mg2+ from the product structure (2w35.pdb) shows two pockets, one for damage recognition (shown for hypoxanthine) and one for incision with a single Mg2+. A helical wedge separates the two pockets.