Table 4.
Cell growth-related genes with expression levels in KYSE170 cells that were changed by the depletion of Na+/K+/2Cl- cotransporter 1
| Gene symbol | Gene ID |
Biological functions |
Fold change | |
| Cell growth and proliferation | Cell cycle | |||
| Upregulated genes | ||||
| NDN | NM_002487 | ● | 66.45 | |
| INPP5D | NM_001017915 | ● | ● | 52.83 |
| CCR1 | NM_001295 | ● | 44.86 | |
| COL1A2 | NM_000089 | ● | 36.72 | |
| EDAR | NM_022336 | ● | 35.86 | |
| RBP4 | NM_006744 | ● | 30.40 | |
| DCLK1 | NM_004734 | ● | 28.95 | |
| RARRES1 | NM_002888 | ● | 22.92 | |
| FMOD | NM_002023 | ● | 22.85 | |
| BARX1 | NM_021570 | ● | 21.47 | |
| MAPK10 | NM_138980 | ● | 20.00 | |
| ADORA2A | NM_000675 | ● | 18.78 | |
| CHRNA7 | NM_001190455 | ● | ● | 18.47 |
| SOX10 | NM_006941 | ● | 18.03 | |
| FGF20 | NM_019851 | ● | 16.73 | |
| FAM5C | NM_199051 | ● | ● | 14.35 |
| MBD2 | NM_015832 | ● | 12.13 | |
| EGF | NM_001963 | ● | ● | 11.50 |
| TLR5 | NM_003268 | ● | 11.35 | |
| TNN | NM_022093 | ● | 10.98 | |
| SLC1A2 | NM_004171 | ● | 10.74 | |
| CD36 | NM_001001547 | ● | 10.43 | |
| CD52 | NM_001803 | ● | 10.34 | |
| NR2E3 | NM_016346 | ● | 10.31 | |
| PLCB1 | NM_182734 | ● | 10.26 | |
| MYCN | NM_005378 | ● | ● | 10.23 |
| ZNF365 | NM_199451 | ● | 10.12 | |
| ERG | NM_004449 | ● | 10.04 | |
| MSH4 | NM_002440 | ● | 10.03 | |
| DMRT1 | NM_021951 | ● | 9.61 | |
| RNF128 | NM_194463 | ● | 9.37 | |
| CD69 | NM_001781 | ● | 8.89 | |
| PDE3A | NM_000921 | ● | ● | 8.58 |
| ACVR1C | NM_145259 | ● | 8.57 | |
| SPI1 | NM_001080547 | ● | ● | 8.55 |
| SH2D3C | NM_170600 | ● | 8.53 | |
| IFNG | NM_000619 | ● | ● | 8.22 |
| MRAS | NM_012219 | ● | 8.20 | |
| MCF2L | NM_024979 | ● | 7.43 | |
| RRAD | NM_004165 | ● | ● | 7.42 |
| E2F5 | NM_001951 | ● | ● | 7.32 |
| BGN | NM_001711 | ● | 7.13 | |
| KIFC1 | NM_002263 | ● | 7.02 | |
| ABCC6 | NM_001171 | 6.98 | ||
| SERPINE1 | NM_000602 | ● | 6.74 | |
| CIITA | NM_000246 | ● | 6.74 | |
| GJB6 | NM_006783 | ● | 6.45 | |
| TP53INP1 | NM_033285 | ● | ● | 6.38 |
| GHRL | NM_016362 | ● | 6.30 | |
| CCNG2 | NM_004354 | ● | ● | 6.29 |
| RORC | NM_005060 | ● | 6.24 | |
| NCF1 | NM_000265 | ● | 6.24 | |
| NFATC4 | NM_001136022 | ● | 6.15 | |
| CHRM5 | NM_012125 | ● | 6.01 | |
| HMOX1 | NM_002133 | ● | ● | 6.00 |
| IL18RAP | NM_003853 | ● | 5.98 | |
| C8orf4 | NM_020130 | ● | 5.95 | |
| L1CAM | NM_024003 | ● | 5.87 | |
| TNFSF8 | NM_001244 | ● | 5.85 | |
| MSMB | NM_002443 | ● | 5.77 | |
| ITPR1 | NM_002222 | ● | 5.77 | |
| ITGAL | NM_002209 | ● | ● | 5.73 |
| INHBA | NM_002192 | ● | ● | 5.53 |
| HEYL | NM_014571 | ● | 5.38 | |
| JAK3 | NM_000215 | ● | ● | 5.30 |
| MMP13 | NM_002427 | ● | 5.23 | |
| NNMT | NM_006169 | ● | 5.06 | |
| BNIPL | NM_138278 | ● | 4.86 | |
| LTC4S | NM_145867 | ● | 4.74 | |
| MMP24 | NM_006690 | ● | 4.72 | |
| MMP1 | NM_002421 | ● | 4.66 | |
| CD19 | NM_001770 | ● | ● | 4.52 |
| ADC | NM_052998 | ● | 4.46 | |
| TGFBR1 | NM_004612 | ● | ● | 4.33 |
| RHOB | NM_004040 | ● | 4.32 | |
| CDKN1C | NM_000076 | ● | ● | 4.30 |
| HOXB13 | NM_006361 | ● | ● | 4.20 |
| IPMK | NM_152230 | ● | 4.07 | |
| BMF | NM_001003940 | ● | 4.06 | |
| VTCN1 | NM_024626 | ● | ● | 4.05 |
| CEACAM1 | NM_001712 | ● | 2.97 | |
| TSSK3 | NM_052841 | ● | 2.00 | |
| Downregulated genes | ||||
| CRHR2 | NM_001883 | ● | -39.78 | |
| C5 | NM_001735 | ● | ● | -39.13 |
| KCNMA1 | NM_001014797 | ● | -38.59 | |
| GRIA1 | NM_000827 | ● | -31.27 | |
| SLC12A2 | NM_001046 | ● | -28.92 | |
| APOA1 | NM_000039 | ● | -25.21 | |
| PRKAR2B | NM_002736 | ● | ● | -24.25 |
| TF | NM_001063 | ● | ● | -22.85 |
| BRCA2 | NM_000059 | ● | ● | -22.04 |
| AURKC | NM_001015878 | ● | -21.69 | |
| PLXNA4 | NM_181775 | ● | -20.54 | |
| TYR | NM_000372 | ● | -20.39 | |
| BACH2 | NM_021813 | ● | -20.01 | |
| KIF14 | NM_014875 | ● | -18.06 | |
| HEY2 | NM_012259 | ● | -18.01 | |
| TMPO | NM_003276 | ● | ● | -15.97 |
| FCGR3A | NM_000569 | ● | -15.18 | |
| ARF6 | NM_001663 | -15.09 | ||
| MYBL1 | NM_001080416 | ● | ● | -14.60 |
| CCNA1 | NM_003914 | ● | ● | -14.29 |
| ESCO2 | NM_001017420 | ● | -14.05 | |
| TOP2A | NM_001067 | ● | ● | -13.91 |
| CENPI | NM_006733 | ● | -13.87 | |
| ATAD2 | NM_014109 | ● | -13.41 | |
| POSTN | NM_006475 | ● | -12.91 | |
| MKI67 | NM_002417 | ● | ● | -12.41 |
| ABCB1 | NM_000927 | ● | ● | -12.15 |
| KIF20B | NM_016195 | ● | ● | -11.53 |
| SPN | NM_001030288 | ● | -11.41 | |
| MAD2L1 | NM_002358 | ● | ● | -11.16 |
| HLA-DPB1 | NM_002121 | ● | -11.12 | |
| SGOL1 | NM_001012410 | ● | -10.91 | |
| RRM2 | NM_001034 | ● | -10.71 | |
| FANCD2 | NM_033084 | ● | ● | -10.55 |
| FANCA | NM_001018112 | ● | ● | -10.31 |
| HDAC2 | NM_001527 | ● | ● | -9.94 |
| NUF2 | NM_145697 | ● | -9.76 | |
| CLSPN | NM_022111 | ● | ● | -9.57 |
| RAD54L | NM_003579 | ● | -9.47 | |
| KLHL13 | NM_033495 | ● | -9.32 | |
| CCNA2 | NM_001237 | ● | ● | -9.13 |
| MCM10 | NM_182751 | ● | ● | -9.11 |
| MCTS1 | NM_014060 | ● | -9.02 | |
| ANLN | NM_018685 | ● | -9.00 | |
| HMGB2 | NM_002129 | ● | ● | -8.86 |
| VPREB1 | NM_007128 | ● | -8.81 | |
| KIF4A | NM_012310 | ● | -8.78 | |
| SPC25 | NM_020675 | ● | -8.75 | |
| ALOX5 | NM_000698 | ● | ● | -8.55 |
| PBK | NM_018492 | ● | -8.20 | |
| TNFRSF11B | NM_002546 | ● | -8.20 | |
| CIT | NM_001206999 | ● | -8.17 | |
| HELLS | NM_018063 | ● | ● | -8.1 |
| CDC45 | NM_003504 | ● | ● | -8.07 |
| DTL | NM_016448 | ● | ● | -8.00 |
| RGS3 | NM_017790 | -7.93 | ||
| TYMS | NM_001071 | ● | ● | -7.87 |
| NDC80 | NM_006101 | ● | -7.86 | |
| ERCC6L | NM_017669 | ● | -7.82 | |
| CENPE | NM_001813 | ● | -7.75 | |
| TTK | NM_003318 | ● | ● | -7.74 |
| SIM2 | NM_009586 | ● | -7.61 | |
| KRT4 | NM_002272 | ● | -7.55 | |
| RAD51AP1 | NM_006479 | ● | -7.55 | |
| LTA | NM_000595 | ● | -7.51 | |
| PAK2 | NM_002577 | ● | ● | -7.50 |
| SLC5A8 | NM_145913 | ● | -7.41 | |
| BLM | NM_000057 | ● | ● | -7.40 |
| NUSAP1 | NM_016359 | ● | -7.36 | |
| JDP2 | NM_130469 | ● | ● | -7.19 |
| CASP3 | NM_004346 | ● | ● | -7.17 |
| NEIL3 | NM_018248 | ● | -7.17 | |
| POLH | NM_006502 | ● | ● | -7.11 |
| KIF20A | NM_005733 | ● | ● | -7.08 |
| MYO7A | NM_000260 | -6.93 | ||
| NRGN | NM_006176 | ● | -6.82 | |
| NCAPG | NM_022346 | ● | ● | -6.78 |
| CDCA8 | NM_018101 | ● | ● | -6.72 |
| CEP55 | NM_018131 | ● | -6.65 | |
| DLGAP5 | NM_014750 | ● | ● | -6.60 |
| CDC25C | NM_001790 | ● | ● | -6.59 |
| ARL2BP | NM_012106 | ● | -6.58 | |
| IL12A | NM_000882 | ● | ● | -6.53 |
| MYH14 | NM_001077186 | ● | -6.52 | |
| SKA1 | NM_001039535 | ● | -6.46 | |
| CASC1 | NM_018272 | ● | -6.44 | |
| HJURP | NM_018410 | ● | -6.42 | |
| TACC3 | NM_006342 | ● | ● | -6.33 |
| ENPP3 | NM_005021 | ● | -6.30 | |
| STIL | NM_001048166 | ● | ● | -6.27 |
| KNTC1 | NM_014708 | ● | -6.26 | |
| NR1I2 | NM_003889 | ● | ● | -6.24 |
| AKR1B10 | NM_020299 | ● | -6.22 | |
| E2F2 | NM_004091 | ● | ● | -6.20 |
| USP47 | NM_017944 | ● | -6.14 | |
| KIF11 | NM_004523 | ● | ● | -6.09 |
| E2F8 | NM_024680 | ● | ● | -6.05 |
| PLK1 | NM_005030 | ● | ● | -6.02 |
| CCDC6 | NM_005436 | ● | -6.00 | |
| ORC6 | NM_014321 | ● | -6.00 | |
| EXO1 | NM_003686 | ● | -5.95 | |
| GPC5 | NM_004466 | ● | -5.94 | |
| GSG2 | NM_031965 | ● | -5.93 | |
| PRC1 | NM_003981 | ● | -5.89 | |
| RAD51 | NM_002875 | ● | ● | -5.78 |
| KIF2C | NM_006845 | ● | ● | -5.71 |
| TNFRSF13C | NM_052945 | ● | -5.70 | |
| BLZF1 | NM_003666 | ● | -5.63 | |
| FEN1 | NM_004111 | ● | -5.51 | |
| PLK4 | NM_014264 | ● | ● | -5.49 |
| HAS2 | NM_005328 | ● | ● | -5.44 |
| PKMYT1 | NM_182687 | ● | -5.40 | |
| BUB1 | NM_001211 | ● | ● | -5.34 |
| BUB1B | NM_001211 | ● | ● | -5.34 |
| NEK2 | NM_002497 | ● | ● | -5.33 |
| IQGAP3 | NM_178229 | ● | -5.27 | |
| SKA3 | NM_145061 | ● | -5.23 | |
| PNN | NM_002687 | ● | ● | -5.20 |
| NTRK3 | NM_001007156 | ● | ● | -5.17 |
| IL25 | NM_022789 | ● | -5.09 | |
| UBE2C | NM_181803 | ● | ● | -5.09 |
| AURKB | NM_004217 | ● | ● | -5.08 |
| CDC6 | NM_001254 | ● | ● | -5.08 |
| CDKN2C | NM_078626 | ● | ● | -5.06 |
| EDN2 | NM_001956 | ● | -5.06 | |
| CDC20 | NM_001255 | ● | ● | -5.05 |
| RRM1 | NM_001033 | ● | ● | -5.05 |
| APC | NM_000038 | ● | ● | -5.04 |
| KIF15 | NM_020242 | ● | ● | -5.03 |
| LMNB1 | NM_005573 | ● | -5.02 | |
| NCAPG2 | NM_017760 | ● | -4.96 | |
| CCNE2 | NM_057749 | ● | ● | -4.94 |
| HMMR | NM_012484 | ● | -4.93 | |
| BRIP1 | NM_032043 | ● | -4.90 | |
| ECT2 | NM_018098 | ● | ● | -4.89 |
| CDT1 | NM_030928 | ● | ● | -4.87 |
| MCAM | NM_006500 | ● | -4.82 | |
| LAG3 | NM_002286 | ● | ● | -4.78 |
| ZWINT | NM_032997 | ● | -4.73 | |
| DCLK2 | NM_001040260 | ● | -4.72 | |
| TRAIP | NM_005879 | ● | -4.71 | |
| SSTR2 | NM_001050 | ● | ● | -4.69 |
| TXK | NM_003328 | ● | -4.65 | |
| TBC1D9 | NM_015130 | ● | -4.63 | |
| IL1RN | NM_173843 | ● | -4.61 | |
| CDCA7 | NM_031942 | ● | -4.56 | |
| STK38 | NM_007271 | ● | ● | -4.56 |
| CDCA5 | NM_080668 | ● | ● | -4.54 |
| E2F7 | NM_203394 | ● | -4.54 | |
| FIGNL1 | NM_001042762 | ● | -4.51 | |
| SMC4 | NM_005496 | ● | -4.50 | |
| CYCS | NM_018947 | ● | -4.48 | |
| FBN1 | NM_000138 | ● | -4.48 | |
| NCAPD3 | NM_015261 | ● | -4.46 | |
| IL16 | NM_172217 | ● | ● | -4.44 |
| PCNA | NM_002592 | ● | ● | -4.42 |
| FBXO5 | NM_001142522 | ● | -4.37 | |
| CKAP2 | NM_018204 | ● | -4.34 | |
| IL34 | NM_152456 | ● | -4.34 | |
| PSRC1 | NM_032636 | ● | ● | -4.33 |
| C11orf82 | NM_145018 | ● | -4.32 | |
| CHRDL1 | NM_145234 | ● | -4.31 | |
| RAD54B | NM_012415 | ● | -4.31 | |
| DIAPH3 | NM_001042517 | ● | -4.29 | |
| AKR1C1 | NM_001353 | ● | -4.26 | |
| INHBB | NM_002193 | ● | -4.25 | |
| MDM2 | NM_002392 | ● | ● | -4.25 |
| PRKAA1 | NM_206907 | ● | -4.25 | |
| MASTL | NM_032844 | ● | -4.23 | |
| MCM5 | NM_006739 | ● | -4.21 | |
| CD2AP | NM_012120 | ● | ● | -4.20 |
| BRCA1 | NM_007300 | ● | ● | -4.18 |
| TPX2 | NM_012112 | ● | ● | -4.15 |
| FGFBP1 | NM_005130 | ● | -4.14 | |
| EIF4G2 | NM_001172705 | ● | ● | -4.12 |
| AURKA | NM_198433 | ● | ● | -4.10 |
| PTTG1 | NM_004219 | ● | ● | -4.08 |
| ADRA1B | NM_000679 | ● | ● | -4.07 |
| RECQL4 | NM_004260 | ● | -4.02 | |
| GJB2 | NM_004004 | ● | -4.00 | |
| BIRC5 | NM_001012271 | ● | ● | -3.35 |
| TERF1 | NM_017489 | ● | ● | -3.18 |
| LRP1 | NM_032832 | ● | -2.43 | |
| CDK1 | NM_012395 | ● | ● | -2.21 |
| ARHGEF10 | NM_014629 | ● | -2.08 | |