Figure 8.

The ACPD effect on myelin protein expression is blocked when astrocyte-derived BDNF is reduced. Cre recombinase + GFAPcreERT2-floxBDNF-ROSA26 mice and Cre recombinase − floxBDNF-ROSA26 controls were injected with tamoxifen and fed cuprizone-laden food for 4 weeks before they received a single stereotaxic injection of either ACPD or saline vehicle. a, Western blots demonstrate MBP and MAG protein in the corpus callosum of these mice. GAPDH is shown as a loading control. b, Graph represents a densitometric analysis of Western blots normalized to GAPDH and are presented as percentage cre- saline-injected control. Western blot data analyzed by ANOVA; *significantly different from saline-injected, cre- controls at p < 0.01; ***significantly different at p < 0.01. Each Western blot lane is from the corpus callosum of a single mouse within one experiment. Each experiment was repeated four times.