Figure 7.

The gross morphology of the cerebellum is unaltered after blocking Purkinje cell GABAergic neurotransmission. A, A mid-sagittal section from a control cerebellum. Purkinje cells are labeled with calbindin (magenta) and the granular layer is particularly highlighted by the fluorescent Nissl stain (green). B, A mid-sagittal section from a mutant cerebellum. Scale bars: A, B, 200 μm. C, An example image showing how the cerebellar cortical measurements were taken from within the primary fissure. Scale bar, 200 μm. D, Quantification of body weight, brain weight, cerebellar weight, and molecular layer thickness. Ml, molecular layer; pcl, Purkinje cell layer; gl, granular layer; ***p < 0.01.