Figure 7.

USP1 Deletion Restores FANCD2 Monoubiquitination but Not Sensitivity to MMC in ΔFANCC Cells

(A) DT40 cell lines with indicated genotypes were treated with MMC, fractionated into cytonucleoplasmic (S) and chromatin (P) fractions, and analyzed by western blotting with anti-FANCD2.

(B) Cellular sensitivity of DT40 cells after exposure to indicated doses of MMC. The mean percentage of survival ± SEM of three independent colony-formation assays are plotted relative to untreated cells.

(C) The FA core complex is comprised of multiple modules. B-L-100 (green) is central to the monoubiquitination step. The A-G (orange) and C-E-F (blue) modules may affect this catalytic function through regulatory or stabilizing roles. They may also serve distinct functions independent of ubiquitination in FA DNA repair. Subunits shown to be dispensable for ubiquitination in vivo are in gray.

See also Figure S6.