Fig. 3.

Biliverdin modulates C5aR expression in part via mTOR signaling. RAW 264.7 Mφ were pre-incubated with rapamycin for 1 h and thereafter treated with BV or LPS for 15 min or 24 h for pS6 and C5aR expression, respectively. (A) Protein expression of pS6 and (B) cell surface expression of C5aR in RAW 264.7 cells. The data are representative of three independent experiments. Value represents mean ± S.E. n = 3/group, ^#P < 0.05 vs. no rapamycin control (0.01% DMSO), ^∗P < 0.05 vs. no rapamycin and no LPS control (0.01% DMSO), ^&P < 0.05 vs. no rapamycin and LPS control and ^&#P < 0.05 vs. no rapamycin BV + LPS group.