Table 1.
Primer sequences and amplicon sizes of housekeeping (GAPDH and HPRT) and target genes (C5aR, TNF-α and IL-6) expressed in RAW 264.7 cells.
| Gene target | Forward sequence | Reverse sequence | Amplicon size (bp) |
|---|---|---|---|
| GAPDH | TCAACAGCAACTCCCACTCTTCCA | ACCCTGTTGCTGTAGCCGTATTCA | 115 |
| HPRT | AGGAGTCCTGTTGATGTTGCCAGT | GGGACGCAGCAACTGACATTTCTA | 134 |
| C5aR | TCATCCTGCTCAACATGTACGCCA | TCTGACACCAGATGGGCTTGAACA | 93 |
| TNF-α | TCTCATGCACCACCATCAAGGACT | ACCACTCTCCCTTTGCAGAACTCA | 92 |
| IL-6 | ATCCAGTTGCCTTCTTGGGACTGA | TAAGCCTCCGACTTGTGAAGTGGT | 134 |