Fig. 7.

‘Kiss-and-run’ granule recapture is increased in the db/db cells. Two extracellular dyes, SRB (pH insensitive) (solid lines) and HPTS (dashed lines) both enter each fusing granule. (a) For most granule fusion events the average fluorescence intensity changes are similar for both dyes. (b) In contrast, some granule fusion events show a selective decrease in the HPTS signal where it is quenched by acidification. Scale bars 1 μm; n = 4 WT and n = 4 db/db mice; total of 580 events