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Schematic figure of the incubation chamber. Full thickness sheets of bladder dome were sandwiched between two separated bathing solutions, with each containing gassed (5% CO2/95% O2) Krebs-bicarbonate (serosal) or DMSO (urothelial) solution at 37°C. Tissues were incubated with DMSO (50% v/v) applied to the luminal side only for 15 min before isolation of the various tissues for pharmacological analysis.
