Figure 1.

Flowering time and the expression of flowering time genes under the end-of-day light quality treatments in the perpetual flowering F. vesca accession Hawaii-4 (H4). (A) Flowering time of the seedlings of H4 indicated as the number of leaves in the primary leaf rosette before the terminal inflorescence (n = 8–11). Flowering results were subjected to One-Way ANOVA (t-test), p < 0.001 for the treatment. Different lower-case letters indicate significant differences between the treatments according to Tukey's pairwise test, α = 0.05. (B) The expression of FvFT1 in the leaves of H4 collected in different time points during the treatments. (C) The expression of FvSOC1, (D) FvAP1, and (E) FvFUL1 in the shoot apex samples of H4 collected in different time points. Plants with one open leaf were subjected to 12-h short-day (SD) or 12-h SD plus 6-h low intensity (8 μmol m⁻²s⁻¹) end-of-day treatment with incandescent, far-red or red light (INC, FR, and R, respectively) at 18°C for 5 weeks. For gene expression data, three biological replicates were analyzed by real-time PCR. Leaf and shoot apex samples were collected 4 h after dawn. All results are mean ± SD.