Figure 2.

The overexpression of FvFT1 causes extreme early flowering in the perpetual flowering F. vesca accession Hawaii-4 (H4). (A) H4 wild type, and (B) 35S:FvFT1 line #7 at the age of 8 weeks. (C) Flowering time of FvFT1 overexpression lines in different end-of-day light quality treatments indicated as the number of leaves in the primary leaf rosette before the terminal inflorescence (#5: n = 21–31, #7: n = 13–18, wild type H4: n = 36–38). Flowering results were subjected to Two-Way ANOVA (p < 0.001 for each of treatment, genotype, and treatment × genotype interaction). Pairwise comparisons were carried out for every genotype separately using Tukey's test, α = 0.01. Treatments with different letters indicate significant differences within the genotype. (D) The expression of FvFT1 in 35S:FvFT1 plants compared to wild type Hawaii-4 under LD (18 h). T₁ and H4 seedlings with open cotyledons were subjected to 12-h short-day (SD) or 12-h SD plus 6-h low intensity (15 μmol m⁻²s⁻¹) end-of-day treatment with far-red, red or blue light (FR, R, and B, respectively) at 22°C for 8 weeks. For gene expression data, three biological replicates were analyzed by real-time PCR. Leaf samples were collected 16 h after dawn. All results are mean ± SD.