Table 3.
Efficacy of HuScFv specific to N. kaouthia long neurotoxin (NkLN-HuScFv) in the cross-neutralization of O. hannah venom.
| Group of mice | Treatment (injected i.p. with) | Ratio of venom : HuScFv (w/w) | Mean ± SD of mouse dead time ( min) | % Survival |
|---|---|---|---|---|
| 1 | Venom-NkLN-HuScFv mixture | 1:10 | 111 ± 16.5 a | 0 |
| 2 | Venom-irrelevant HuScFv mixture | 1:10 | 88 ± 7.2 b | 0 |
| 3 | Venom-NkLN-HuScFv mixture | 1:50 | 155 ± 58.7 a | 0 |
| 4 | Venom-irrelevant HuScFv mixture | 1:50 | 93 ± 6.8 b | 0 |
| 5 | Venom in buffer | NA | 78 ± 7.3 b | 0 |
| 6 | NKLN-HuScFv alone | NA | All mice survived at the end of experiments | 100 |
| 7 | Venom-NkLN-HuScFv mixture and followed 10 min later with NkLN-HuScFv alone | 1:50 | 220 a | 80 |
| 8 | Venom-irrelevant HuScFv mixture and followed 10 min later with irrelevant HuScFv alone | 1:50 | 95 ± 24.4 b | 0 |
Notes: NA, not available. Entries with different superscripts (a versus b) were different significantly at p < 0.05 (t-test). Irrelevant HuScFv were specific to the influenza A virus M1 protein.