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. 2014 May 20;106(10):2105–2114. doi: 10.1016/j.bpj.2014.04.017

Figure 2.

Figure 2

Replacement and incorporation of the adenoviral transduced adult cardiac myocytes. (A) Representative Western blot of adult rat ventricular myocytes day 3 after transduction probed with a pan-TnI antibody. Flag-cTnI, endogenous cTnI and ssTnI labels denote expected sizes for each isoform. All cTnI isoforms are Flag tagged. (B) Quantification of TnI replacement based on Western blot comparing exogenous (Flag band) to total TnI (n = 9–12 for each group from 3 to 4 independent experiments). (C) Indirect immunofluorescence of FLAG-tagged substitutions showing normal sarcomeric staining. Measurement bar = 10 μm. Inset shows normal thin filament staining for the two substitutions cTnI AH and cTnI QAEH compared to WT cTnI. To see this figure in color, go online.