Figure 7.

Relative Ca²⁺ binding free energy. The change in Ca²⁺ binding free energy normalized to the average of WT cTnI plotted as, $\Delta \Delta G_{bind}^{wild\to mut}$. Four representative frames from all simulations for a given isoform were calculated and averaged, error bars are +/− SE. ^∗p < 0.05 from a Student’s t-test to cTnI value.