Figure 2.

Confirmation of mutation by SDS-PAGE. Samples were run in a 10–20% gradient gel, and stained with Coomasie Brilliant Blue R-250. The β-γ fusion mutant and the protrusionless mutant were each run in three lanes, where – – indicates purified subcomplex before thrombin treatment, + – after thrombin treatment for 10 min (reaction mixture), and + + the subcomplex after further purification by size-exclusion chromatography. γΔpro, the γ subunit of the protrusionless mutant.