Table 1.

Number of 0.29-μm beads observed on surfaces with the WT or protrusionless TF₁

	Rotating duplexes	Fluct. duplexes	Stuck duplexes	Higher aggre.	Single beads	Total
Wild (1 nM)
Ch 1	38	191	109	374	958	1670
Ch 2	31	132	73	149	909	1294
Ch 3	27	140	70	130	700	1067
Ch 4	31	144	62	163	622	1022
Ch 5	29	65	31	51	460	636
Ch 6	19	80	48	53	552	752
Mean	29.2	125.3	65.5	153.3	700.2	1073.5
SD	6.2	46.1	26.4	118.2	197.9	374.7
%Total	2.7%	11.7%	6.1%	14.3%	65.2%	100%
Protrusionless (5 nM)
Ch 1	16	288	281	616	1726	2927
Ch 2	6	135	97	159	949	1346
Ch 3	14	108	138	142	764	1166
Ch 4	10	141	174	247	977	1549
Ch 5	14	141	162	186	1362	1865
Ch 6	11	129	125	113	1038	1416
Mean	11.8	157.0	162.8	243.8	1136.0	1711.5
SD	3.6	65.3	64.0	187.9	348.5	639.7
%Total	0.7%	9.2%	9.5%	14.3%	66.4%	100%

For each chamber (Ch 1 to 6), randomly selected 18 fields of view (31 × 31 μm²) were examined for 12 s at 2 mM ATP. Some beads stopped or started rotation during the observation period, and these were counted as rotating. Fluctuating and stuck duplexes were distinguished by eye. Higher aggregates and singles were counted as such irrespective of whether moving or not. Without TF₁, 13 beads were found per chamber.