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A transmural gradient in Ito. (A) Representative traces of outward currents from five transmural layers. (B) Peak Ito current-voltage relationships exhibit a transmural gradient in Ito magnitude at test potentials >20 mV. (C) The Ito gradient can be attributed to changes in the amplitude and not the voltage dependence of Ito. (D) A2 does not shift the voltage dependence but only affects the amplitude of Ito.
