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(a) Average SHG signal showing the dramatic drop in signal on the anodic pole at the pulse application (2 s). (b and c) Simultaneous TPF signals showing nearly no instantaneous change at the pulse application. (d) GP showing no observable changes in the membrane potential or fluidity after the pulse. (Shaded areas) Fit to the mean ± SE for each trace (n = 6). (e) Conceptualization of the hypothesized membrane disruption underlying the observed change in SHG response.
