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. 2014 May 20;106(10):2085–2095. doi: 10.1016/j.bpj.2014.03.049

Figure 1.

Figure 1

tBid forms oligomers in the membrane of mitochondria-like liposomes. (A and B) FRET efficiency calculated from the DAC fluorescence and corrected for environmental effects using the signal obtained in the absence of acceptor (A) and normalized NBD fluorescence (B) measured after addition of 100 nM acceptor cBid-NBD to 20 nM donor cBid-DAC premixed with liposomes. Each curve corresponds to the average of three repeats. Lines are single (B) or multiple (A) exponential fits of the data. (C) Comparison between the half-times associated with the insertion of Bid residue 126 (solid triangles and squares) or 163 (solid inverted triangles) in the membrane and those associated with tBid oligomerization (open circles), as obtained from the exponential fit of either NBD-fluorescence or DAC-NBD FRET data as shown in A and B (mean ± SD, n = 3). Data shown as squares and circles are from this study, whereas data shown as triangles and inverted triangles was taken from Shamas-Din et al. (11). (D) Simple model for the interaction of cBid with a lipid membrane. To see this figure in color, go online.