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. 2014 Jun 3;106(11):2395–2407. doi: 10.1016/j.bpj.2014.04.016

Figure 4.

Figure 4

LPS concentration and time dependence on hole formation. LSCM fluorescence images of a DOPC sLBA treated for 20 min with LPS at varying concentrations of LPS in PBS: (A) 500 μg/mL, (B) 100 μg/mL, or (C) 20 μg/mL, and then washed with PBS, showing decreasing numbers of holes with LPS concentration. Parallel samples were treated with 5 μg/mL LPS in PBS for (D) 20 min, (E) 60 min, or (F) 180 min, and then washed and imaged immediately. More holes are observed with increased incubation time. These images were acquired at higher pixel density (4096 × 4096) to resolve small holes. (D–F, inset) Digitally magnified areas of these images showing small holes more clearly. To see this figure in color, go online.