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. 2014 Jun 2;13:136. doi: 10.1186/1476-4598-13-136

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Copyright © 2014 Li et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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An Akt/ERK-miR-200c-ZEB2 axis was involved in IGF-induced EMT in gastric cancer cells. MGC-803 and SGC-7901 cells were serum-starved overnight and then treated with or without 100 ng/mL of IGF-I for 48 h. (A) The expression of ZEB2 was analyzed by Western blot. (B) The level of mRNA for ZEB2 was analyzed by RT-PCR. (C-E) The serum-starved cells were pretreated with or without PD98059 (20 μM) or LY294002 (100 μM) for 2 h followed by IGF-I (100 ng/mL) stimulation for 48 h. The expression of miR-200c was analyzed by real-time PCR. Data are means ± SD in three independent experiments. * IGF-I untreated vs. IGF-I treated, p < 0.05. Control group as reference. PD, PD98059; LY, LY294002.