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. 2014 Apr 29;12(5):2341–2356. doi: 10.3390/md12052341

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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NPCs^fat-1 attenuated H₂O₂-mediated apoptosis. (A) The cell viability of NPCs was assessed after exposure to H₂O₂ for 6 h by WST-8 analysis. Each value represents the mean ± SD of three independent experiments (n = 3, ** p < 0.01 versus other groups); (B–E) Representative photomicrographs of TUNEL assay; (F) Quantitative analysis was carried out by measuring TUNEL-positive cells in each group. Figures were selected as representative data from three independent experiments. Cell apoptosis was significantly reduced in DHA-pretreated NPCs^WT and NPCs^fat-1. Each value represents the mean ± SD of three independent experiments (n = 3, ** p < 0.01 versus other groups). Scale bar: 75 μm.