Figure 4.
Expression analyses of Nrf2-ARE pathway genes in DHA-pretreated NPCsWT and NPCsfat-1. Real-time RT-PCR assays showed that both DHA pretreatment and NPCsfat-1 induced significant increases in the transcript level of Nrf2 and its downstream gene and phase II detoxification gene transcripts HO-1, NQO-1, GCLC, GCLM when compared with the controls. Data are shown as mean ± SD (n = 3); * p < 0.05 versus control; ** p < 0.01 versus control.