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. 2014 Apr 29;12(5):2341–2356. doi: 10.3390/md12052341

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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DHA pretreatment and NPCs^fat-1 induced a significant increase in nuclear Nrf2 expression and a significant decrease in cytosolic Nrf2 expression. DHA-pretreated NPCs^WT and NPCs^fat-1 were treated with 200 μM H₂O₂ for the indicated time points. Cells were lysed and fractionated to isolate nuclear and cytosolic fractions as indicated. Fractions were confirmed using Western blot with histone H3 for nuclear fractions (A) and β-actin as a marker for cytosolic fractions (B); Densitometry analysis showed that DHA pretreatment and NPCs^fat-1 induced a significant increase in nuclear Nrf2 expression (C) and a significant decrease in cytosolic Nrf2 expression (D). Data are shown as mean ± SD (n = 3); * p < 0.05 versus control; ** p < 0.01 versus control.