Typical patterns of aggregation with washed platelets. (A,B) Washed platelets (WP) were incubated for 2 min at 37 °C while stirring with 0.173 mM (Line b), 0.347 (Line c) and 0.694 (Line d) of Pachydictyol A/isopachydictyol A, then 16 μg/mL collagen (upper panel) or 10 nM thrombin (lower panel) was added to induce aggregation. (C,D) WP were incubated as above with dichotomanol (0.34 mM), and then collagen (upper panel) or thrombin (lower panel) was added to the medium. For all panels, Lines a represent WP incubated with 1% DMSO (v/v, final concentration). The arrows mark the addition of agonists.