Table 1.

The effect of diterpenes on coagulation time analyzed by different methods. For prothrombin time (PT), diterpenes were incubated with plasma for 10 min at 37 °C, and then thromboplastin was added to induce coagulation. For the activated partial thromboplastin time (aPTT), diterpenes were incubated with plasma plus cephalin for 10 min at 37 °C, and then, CaCl₂ (8.3 mM) was added to induce coagulation. For fibrinogen coagulation (FC), diterpenes were incubated for 10 min at 37 °C with fibrinogen (2 mg/mL), and then, thrombin (10 nM) was added to induce coagulation. Results are expressed as the means ± SEM of two individual experiments (n = 8). PAC/ISO, pachydictyol A/isopachydictyol A; DIC, dichotomanol. * p < 0.05 when compared with NaCl or DMSO.

Samples	Concentration	Coagulation time (s)
		PT	aPTT	FC
NaCl	150 mM	21.6 ± 0.5	62.90 ± 1.3	26.4 ± 0.5
DMSO	1% (v/v)	23.3 ± 0.3	65.75 ± 1.0	34.1 ± 1.4
	2% (v/v)	26.7 ± 0.4	73.6 ± 1.8	40.8 ± 1.3
PAC/ISO	0.7 mM	23.9 ± 1.0	72.8 ± 1.5 *	65.3 ± 3.5 *
	1.4 mM	26.9 ± 0.7	87.6 ± 0.9 *	66.7 ± 0.8 *
DIC	0.7 mM	32.4 ± 1.8 *	101.8 ± 4.9 *	47.3 ± 1.6 *
	1.3 mM	41.0 ± 2.6 *	139.9 ± 5.8 *	86.4 ± 2.6 *