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Effect of temperature (a) and pH (b) on the agarolytic activity and stability of the recombinant β-agarase YM01-3. Temperature profiles were measured at different temperatures (0–100 °C) in 20 mM Tris-HCl buffer (pH 8.0). pH profiles were measured at 50 °C in different buffers. All data shown are the mean values from at least three replicate experiments.
