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. 2014 May 22;12(5):3072–3090. doi: 10.3390/md12053072

Figure 1.

Figure 1

A furanoterpenoid derivative, 10AB, induces apoptosis in HL 60 cells. The cells were treated with different doses of 10AB (0, 1.5, 3.0, and 6.0 μM) for 24 h. (A) The treated cells were stained with DAPI. The morphological changes were examined with fluorescence microscopy; (B) The treated cells were stained with annexin-V/PI and examined with flow cytometry. The results are presented as means ± SD of three independent experiments and ** p < 0.001 indicats statistically significant differences compared with the control group (DMSO treatment); (C) Cells were pretreated with or without 25 μM of Z-VAD-FMK and then treated with 6.0 μM of 10AB for 24 h. Cell lysates were analyzed via immunoblotting with specific antibodies; (D) The viability of normal rat alveolar macrophage NR8383 cells was determined with different doses of 10AB (0, 1.5, 3.0, and 6.0 μM).