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The effect of 10AB on topo IIα function. (A) The effect of 10AB on topo IIα mediated supercoiled pHOT1 plasmid DNA relaxation with cell-free system; (B) 10AB decreased the expression of topo IIα protein in HL 60 cells. HL 60 cells were treated with 10AB (0, 1.5, 3.0 and 6.0 μM) for 24 h. The protein expression of topo IIα was analyzed via Western blotting. The bands were quantified via densitometry and normalized relative to β-actin levels.
